Touchdown pcr faq12/24/2023 Panel: TOPO Tools: Creating Linear Expression Constructs with Functional Elements227.Panel: BioBricks and the Ordered Assembly of DNA Fragments225.Panel: A Guide to Cloning the Products of Polymerase Chain Reactions218.Panel: The History of Transformation of Bacteria by DNA217.coli: Transformation and Storage of Bacterial Cells in the Same Solution175 Panel: Alternative Protocol: One-Step Preparation of Competent E.Panel: Cloning and Transformation with Plasmid Vectors157.Protocol 14: Screening Bacterial Colonies Using X-Gal and IPTG: -Complementation.Protocol 13: Cloning PCR Products: TOPO TA Cloning.Protocol 12: Cloning PCR Products: TA Cloning.Protocol 11: Cloning PCR Products: Making T Vectors.Protocol 10: Cloning PCR Products: Blunt-End Cloning.Protocol 9: Cloning PCR Products: Addition of Restriction Sites to the Termini of Amplified DNA.Protocol 8: Attaching Phosphorylated Adaptors/Linkers to Blunt-Ended DNAs.Protocol 7: Dephosphorylation of Plasmid DNA.Protocol 6: Cloning in Plasmid Vectors: Blunt-End Cloning.Protocol 5: Cloning in Plasmid Vectors: Directional Cloning.coli: Nanoparticle-Mediated Transformation Protocol 2: The Inoue Method for Preparation and Transformation of Competent E.Protocol 1: The Hanahan Method for Preparation and Transformation of Competent E.Chapter 3: Cloning and Transformation with Plasmid Vectors157.Panel: Formamide and Its Uses in Molecular Cloning153.Panel: Additional Protocol: Stripping Probes from Membranes150.Panel: Additional Protocol: Autoradiography of Alkaline Agarose Gels117.Protocol 13: Southern Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Membranes.Protocol 12: Southern Blotting: Simultaneous Transfer of DNA from an Agarose Gel to Two Membranes.Protocol 10: Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method.Protocol 9: Recovery of DNA from Low-Melting-Temperature Agarose Gels: Organic Extraction.Protocol 8: Recovery of DNA from Agarose Gels Using Glass Beads.Protocol 7: Imaging: Autoradiography and Phosphorimaging.Protocol 6: Alkaline Agarose Gel Electrophoresis.Protocol 5: Detection of DNA in Polyacrylamide Gels by Autoradiography.Protocol 4: Detection of DNA in Polyacrylamide Gels by Staining.Protocol 3: Polyacrylamide Gel Electrophoresis.Protocol 2: Detection of DNA in Agarose Gels by Staining.Protocol 1: Agarose Gel Electrophoresis.Panel: Minimizing Damage to Large DNA Molecules77.Panel: DNA Extraction from Formaldehyde-Fixed Tissue Embedded in Paraffin Blocks72.Panel: Alternative Protocol: One-Tube Isolation of DNA from Mouse Tails62.Panel: Alternative Protocol: Isolation of DNA from Mouse Tails without Extraction by Organic Solvents61.Panel: Commercial Kits for Purification of DNA3. Panel: Isolation and Quantification of DNA1.Protocol 19: Quantifying DNA in Solution with PicoGreen.Protocol 18: Estimating the Concentration of DNA by Fluorometry Using Hoechst 33258.Protocol 17: Using Ethidium Bromide to Estimate the Amount of DNA in Bands after Electrophoresis through Minigels.Protocol 16: Rapid Isolation of Yeast DNA.Protocol 15: Preparation of Genomic DNA from Mouse Tails and Other Small Samples.Protocol 14: A Single-Step Method for the Simultaneous Preparation of DNA, RNA, and Protein from Cells and Tissues.Protocol 13: Isolation of High-Molecular-Weight DNA from Mammalian Cells Using Proteinase K and Phenol.Protocol 12: Isolation of High-Molecular-Weight DNA Using Organic Solvents to Purify DNA.Protocol 11: Preparation of Double-Stranded (Replicative Form) Bacteriophage M13 DNA.Protocol 10: Growing Bacteriophage M13 in Liquid Culture.Protocol 8: Preparation of Single-Stranded Bacteriophage M13 DNA by Precipitation with Polyethylene Glycol.Protocol 7: Concentrating Nucleic Acids by Extraction with Butanol.Protocol 6: Concentrating and Desalting Nucleic Acids with Microconcentrators.Protocol 5: Precipitation of DNA with Isopropanol.Protocol 4: Precipitation of DNA with Ethanol.Protocol 3: Isolating DNA from Gram-Negative Bacteria (e.g., E.Protocol 2: Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreps.Protocol 1: Preparation of Plasmid DNA by Alkaline Lysis with SDS: Minipreps.Chapter 1: Isolation and Quantification of DNA1.Table of Contents Expand All | Contract All
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Short pampas grass decor12/24/2023 It is a challenging process that will leave you with sore feet and muscles, no matter how careful you are. We understand, pruning pampas grass is difficult and takes time. Therefore, if you plan on growing some pampas grass, get ready to prune it and cut it back at least once every year. The effects of its invasiveness are such severe point that some countries have banned it. Pampas grass grows very fast and is invasive, and if left without care, it can suffocate flowers, trees, or any plant on the way. When pruned, your pampas grass ends up being more robust and more beautiful.Ĭutting pampas grass eliminates the ability to outgrow other plants around it. It is going to keep your plants neat and organized and encourage new growth. Pruning is one of the most basic ways to take care of your plants. Doing so helps maintain their height, not allowing them to overgrow. It is essential to prune and cut all kinds of plants, including flowers and ornamental grasses. You can prune in late spring or in the fall. If you don’t want to keep the foliage, you don’t have to wait until late winter or early spring. Once dry, take it down and use it in any way you like. Spray them with hair spray to ensure the seeds stay attached. Hang the foliage upside down in a dry and dark location for a week or so. If you plan to keep any foliage you trim, you will need to prepare it before use. In this season, the plumes and leaves are openly giving you feathery-like flowers. The best time to trim your plant is late winter or early spring if you want to keep the foliage and use it for décor.
A call to arms runeword12/24/2023 The seven assistants opened the subspace and took out the skeletons one by one. “I will explain the rules of practice from now on.” The head assistant bowed respectfully toward the twins and looked at them with solemn eyes. The twin professors laughed like bad boys.Īs soon as she had finished her words seven teaching assistants came forward to the students with serious faces. The students flinched and stepped back. “At least drawing on paper or something!” “Hey wait a minute! Do you have any practice?” “However if you are dealing with an enemy stronger than a ghoul and the number of opponents is high it is more reasonable to strengthen the ghoul and reduce the number of enemies than to lose the ghoul to the enemy’s blade in vain.”Ī very necromancer-esque way of thinking and the students nodded their heads in agreement.Ĭries came out from everywhere at the twin professor’s ‘Practice Declaration’ that jumped out right away. “Because ghouls aren’t undead like zombies it’s true that there are likes and dislikes about Reno’s golden line even among necromancers.” The undead at the top tier of zombies are ghouls. In fact it is not an exaggeration to say that they are undead of the same class.Īnd if Zombie Corpse Explosion is a skill that overloads the summoning magic circle and makes it explode like a bomb Ghoul’s Funeral is a form of greatly strengthening the power for a while instead of drying up the summoning magic circle. ‘Is it like a ghoul version of a corpse explosion?’ Simon crossed his arms with a serious face. “Rino’s golden ship is a runaway machine prepared for the sacrifice of a ghoul.” The ghoul that the two professors had been dealing with just now stood still and hardened then stopped moving.Įven as an undead it was completely destroyed. The eyes of the students belatedly turned to the magic circle that was spread out in the air.Īfter the jet-black which should be circulating passed through a one-way street the magic circle dried up and lost its vitality like a withered flower. Following the formula the main rune word finally dried up and the entire magic circle scattered into the air and disappeared. “But if you pour jet black like that and strengthen your whole body the ghoul will…” The twin professors spread their palms and said ‘Stop!’ I shouted and the ghoul finally stopped. However as if he couldn’t control his strength he wriggled his body intermittently even when he stopped! wiggle! looked likeĪseraj who was in 5th place raised his hand and asked if he was surprised. The ghoul swings his arm but a tree jumps! crashed with a sound The runaway ghouls turned the surroundings into a mess and the assistants seemed accustomed to supporting trees or protecting equipment. The moment I sent jet black and drew a line on the magic circle drawn on the ghoul’s skull the ghoul screamed and ran across the clearing at such a speed that it left an afterimage. They demonstrated with the ghouls they had brought out beforehand.Ī ghoul that had been jumping around and playing since earlier. “The ghouls affected by this funeral song will be able to exert three to four times their normal strength.” “This is the forced runaway of the ghouls using the ‘Golden Line of Reno’.” The moment when their line passes through the last point called ‘Rex’ and exits the border of the magic circle.Ī large amount of jet-black emanated from the outside and transmitted to the whole body of the ghoul. Just like the rivers that each flowed eventually converge to the sea all pitch black will pass through this line.” Rin on the left crossed her fingers. She moved the magic circle on her screen as she moved her finger.Īll the jet-black elements of the magic circle that maintained the ecosystem in perfect balance gathered together in the jet-black line she drew and began to flow. Strikethrough google docs alt code12/24/2023 It can take the role of a standalone script, a plugin for Blosxom or a Movable Type, or of a text filter for BBEdit. Gruber wrote a Perl script,, which converts marked-up text input to valid, well-formed XHTML or HTML and replaces angle brackets ( ) and ampersands ( &) with their corresponding character entity references. Its key design goal was readability, that the language be readable as-is, without looking like it has been marked up with tags or formatting instructions, unlike text formatted with 'heavier' markup languages, such as Rich Text Format (RTF), HTML, or even wikitext (each of which have obvious in-line tags and formatting instructions which can make the text more difficult for humans to read). Gruber created the Markdown language in 2004, with Swartz acting as beta tester, with the goal of enabling people "to write using an easy-to-read and easy-to-write plain text format, optionally convert it to structurally valid XHTML (or HTML)." In 2002 Aaron Swartz created atx and referred to it as "the true structured text format". Markdown was inspired by pre-existing conventions for marking up plain text in email and usenet posts, such as the earlier markup languages setext (c. This was addressed in 2014 when long-standing Markdown contributors released CommonMark, an unambiguous specification and test suite for Markdown. The initial description of Markdown contained ambiguities and raised unanswered questions, causing implementations to both intentionally and accidentally diverge from the original version. Markdown is widely used for blogging and instant messaging, and also used elsewhere in online forums, collaborative software, documentation pages, and readme files. John Gruber created Markdown in 2004 as a markup language that is easy to read in its source code form. Markdown is a lightweight markup language for creating formatted text using a plain-text editor. Pandoc, MultiMarkdown, Markdown Extra, CommonMark, RMarkdown ĭaringfireball. Hand privacy eraser12/24/2023 Improved localization and language support. Improved Microsoft Edge History cleaning. Crack?Īdded cleaning for Microsoft Store apps: Amazon Music, Disney+, Microsoft To Do, Netflix and VLC Media Player. You can set Privacy Eraser to run automatically every time you close your browser, or you can set it to monitor your Windows system in the background and detect when to clean.Removes obsolete and invalid registry entries for non-existing applications, fixes corrupted registry entries, optimizes your PC’s performance and speeds up Windows startup.Latest Version: 6.0.5 ( What's New) File Size: 9. 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Fifth harmony work from home vevo12/24/2023 While it’s too soon to say if this will mark the dissolution of Fifth Harmony, the group appears to be burning out fast and bright on a funeral pyre of incendiary press releases and passive aggressive tweets. We wish her well.” Note the perfection of that first sentence, with the soon-to-be-iconic phrase “informed via her representatives.” Where was this artistry in any of Fifth Harmony’s singles? In a lengthy missive, the remaining Fifth Harmony members shared that, “After 4 and a half years of being together, we have been informed via her representatives that Camila has decided to leave Fifth Harmony. The news came courtesy of the Notes app, an unsung hero of 2016. That honor goes to Monday’s announcement that Camila Cabello is abandoning ship, heading for the arguably clearer waters of a solo career. In a rare burst of activity for the girl group’s Google Alert, Fifth Harmony’s biggest story this December was neither Jauregui’s drug bust nor her leaked tape. Her attorney told Page Six that, “She will address the matter appropriately through the legal system.” The singer, who was en route to a performance in Brazil, got off with a citation. Just a week before her tape hit the internet, Jauregui was busted for marijuana possession at Washington Dulles International Airport. Luckily, Lauren appears to have found a way to cope with the pressure-pot. Jauregui recently started crying during a concert, later offering the bland explanation that their lyrics made her “extra emotional.”īut just how stressful is this job? Apparently, pretty freaking stressful.įar be it from me to question Simon Cowell’s management style, but being forced to Skype into a loved one’s funeral seems highly abnormal. Over the past few months, the group has cancelled multiple concerts after members appeared to break down onstage. This isn’t the first time Jauregui and her bandmates have voiced concerns over their demanding schedules. In the background, “Ally” can be heard comforting her friend, repeating the phrase “I know” over and over again. “They are making decisions on a regular basis to fuck us over, to make us literal slaves, like literally slaves, Ally,” says the voice, which sounds like Lauren. On Tuesday, a tape leaked that appeared to feature band member Lauren Jauregui complaining to fellow harmonizer Ally Brooke Hernandez. Ironically, the women behind this ode to half-assed employment are actually acute victims of late-stage capitalism. Technically, they weren’t even that popular-the group didn’t achieve a top 10 single on the Billboard charts until 2016’s “Work from Home,” a song that I thought was about freelancing, but is actually about sexual intercourse. With the correct number of women and the requisite amount of diversity, Fifth Harmony didn’t explode onto the scene so much as they orbited around it, backed by an allegedly passionate fan base that I can neither confirm nor deny. In a musical landscape of interchangeable teenagers and endless electronic noise, Fifth Harmony actually sticks out as one of the most formulaic, least distinctive fivesomes out there. Forged in the furnace of a televised talent competition, Fifth Harmony attempted to fill the Spice Girl-sized girl group void-to decidedly meh results. In 2012, a new singing group formed on the second season of The X Factor USA. Nfs most wanted 2021 release date12/24/2023 The series has always been known for its arcadey racing gameplay as opposed to the more realistic simulation games such as Gran Turismo or Forza Motorsport. According to the company, “Need For Speed Most Wanted features stunning dynamic physics-based collisions, exciting cop takedowns, and realistic visual damage.” The physics-based collisions would probably help a great deal with the gameplay, thanks to the ability to execute Takedowns. The trailer shows that the game will feature good graphics, along with the same gameplay that will be there in the other versions of Most Wanted, including the Takedown mechanic, which was originally featured in the Burnout series. EA had made the announcement along with a trailer that shows some of the gameplay on the mobile versions of the game. The game also features a progression system where you can level up to unlock mods for your car, as well as complete challenges such as finishing a race with no tyres to unlock License Plates that will display when you take down an opponent.Įarlier this month, it was revealed that the iOS and Android version of Most Wanted will be released later this month. To kick off multiplayer events, players have to drive to Race Lists strewn across the city that have a variety of different game modes, from simple races to all out takedown battles to jumping competitions. The PC version will see an even later release on November 9.ĮA had also recently released a trailer for Need for Speed: Most Wanted that explained the multiplayer of the game, including what game modes it’ll feature. The game will be available on the Xbox 360 on November 2, while the PlayStation 3 and PlayStation Vita versions of the game will be available on November 5. Though nothing new in the video has been shown, it does show off some of the graphical fidelity that the game is capable of as your car travels at breakneck speeds and takes down opponents’ vehicles. Should you need to re-enable the dedicated GPU, simply follow these steps but instead of disable this device, it should say enable this device.National Games 2023: Services clinch 10 gold medals on penultimate day to put pressure on leaders Maharashtra National Games 2023: Palak Gulia, Rutuja Bhosale and Angad Vir Bajwa add second gold to their tally Then restart your computer and the game should only see one GPU and start on the dedicated, allowing for much better performance. Right click on your integrated graphics and then select disable this device. from there find the display adapters in the list and click on it. Open the start menu and search for device manager. if you have none of those, you can just leave the integrated disabled. This one gives better performance, and the only cost is the monitors plugged directly into your motherboard. Once it was added, I clicked on it, clicked options, and set it to whatever the integrated GPU is called, for me it was power saving mode. In graphics settings, I clicked browse and then found the Need for Speed application in my steam library. The way I did it was to right click on the desktop and pick display settings, then scroll all the way down to graphics settings. This solution does result in worse performance. The first one is to just have Need for Speed launch on your dedicated graphics, or to disable your dedicated graphics temporarily for while you are playing the game. There are two solutions to this, and I will go over how to have both of them work (in Windows 10). The first GPU seen by the computer is the integrated one, so that is what the game picks as the graphics to use, but the computer detects that it is a game and tries to launch it on the dedicated GPU, but the game does not recognize it. Need for Speed Most Wanted only looks for one GPU, because it is a port of a console game. My PC has 2 graphics processors, one integrated into the CPU, and one dedicated GPU. This worked for me, but your mileage may vary. Tertiary colors definition and examples12/24/2023 Understanding secondary colors will help you develop a firmer grasp of color, no matter what industry you’re working with color in. In this post, we’ll explain how color theory applies to secondary colors, how secondary colors relate to the other types of colors on the color wheel, and answer the question, “what are the secondary colors?” In this example of directional lines in art, Artemisia Gentileschi uses both diagonal lines to show the energy and excitement of the scene and vertical lines (in Judith’s attendant) to show strength.Here’s everything you need to know about secondary colors.Īfter learning about primary colors, the next step is to understand secondary colors. Horizontal and vertical lines add stability and strength to an artwork. Diagonal lines create movement an energy. The direction of a line can dramatically alter an artwork. The great variety of line types make them an especially useful tool in artworks. Lines may be continuous or broken, and can be any width or texture. Line is an element of art defined as the path of a point moving through space.
Eddie redmayne fantastic beasts12/24/2023 Focusing too much on just the one thing quickly gets bland. Grindelwald underestimating him and his special knowledge the first time was his major advantage to balance the playing field.Ĭombining plots/themes together is a major strength in my opinion, and is what makes some franchises / fictional universes so much more interesting, when they're actually fleshed out and varied and have all different sorts of things going on and the feeling of a real larger society existing. To be honest I expected it to be kind of boring with just a focus on him and animals and didn't see it for a long time because of it, but I think part of what made it so interesting was he was such an unusual character to throw up against a mystery like Grindelwald, bumping into the plot accidentally.īeing used as an actual agent chasing after him in the second movie doesn't work so well. r/Fantasy Book Database: All lists in one spreadsheet!.The moderation team will review to verify whether or not the post breaks our other rules. This is a placeholder rule that allows users to report posts that they feel are rule-breaking despite being unsure which specific policy a post is breaking. See the wiki for the full text of this rule. Original writing, writing/publishing advice, and worldbuilding help belong in our Writing Wednesday threads. Please read our full rules prior to posting any reviews or articles from blogs or similar channels. 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Please see our full list of what is allowed and modmail us with questions before engaging in these types of things. People who are mainly here for promotion or to rack up karma will be penalized. Promotional Content & Karma FarmingĬommunity comes first. We ask all users help us create a welcoming environment by reporting posts/comments that do not follow the subreddit rules. Do not engage in hate speech, harassment, arguing in bad faith, sealioning, or general pot stirring. Be KindĮvery interaction on the subreddit must be kind, respectful, and welcoming. This also applies to you posting on behalf of your friend/family member/neighbor. Personal benefit includes, but is not limited to: financial gain from sales or referral links, traffic to your own website/blog/channel, karma farming, critiques or feedback of your work from the community, etc. Interactions should not primarily be for personal benefit. Interact with the community in good faith. Respect for members and creators shall extend to every interaction. Visionīuild a reputation for inclusive, welcoming dialogue where creators and fans of all types of speculative fiction mingle. We reserve the right to remove discussion that does not fulfill the mission of /r/Fantasy. We welcome respectful dialogue related to speculative fiction in literature, games, film, and the wider world. r/Fantasy is the internet’s largest discussion forum for the greater Speculative Fiction genre. For updated information regarding ongoing community features, please visit 'new' Reddit. Resource links will direct you to Wiki pages, which we are maintaining. Please be aware that the sidebar in 'old' Reddit is no longer being updated with information about Book Clubs and AMAs as of October 2018. Taptap send spark capital 13.4m12/24/2023 With a formal market size of >700B alone, we’ve got a lot more saving to do for our users. Its first product, Taptap Send, allows immigrants to send money home instantly and with no fee, saving money in the process. While this has affected wealthier countries as well, it has been nothing short of a revolution for the emerging markets where the overwhelming majority of population had been unable to access digital finance previously. Launched in 2019, Taptap is tapping into a transformative shift in global finance: from banks to phones. Read more about the journey in Reid Hoffman’s words or TechCrunch article Taptap Send is backed by top VCs (Spark, Canaan, Reid Hoffman, Breyer Capital, etc.), rapidly growing and a great place for those looking for both impact and a fast-paced tech startup environment. > Team as global as the mission, >30 languages from 25 countries (i.e., if you’re interested in learning to make Chicken Moambe, we’re the place). > Driven by mission, guided by values (see below) – in deed, not just word. > Top performers from top and varied organizations (e.g., Bain, Bolt, Google, McKinsey, SafeBoda, Uber, Zipline) - we like moving fast, and we hire people that do as well. > Founded by Harvard grads / 3rd-time founders - also founded Segovia (successful exit) and GiveDirectly (>300M revenue, fastest-growing NGO). Reduce inequity by helping immigrants move money home by becoming the leading cross-border fintech for immigrants. AuthorWrite something about yourself. 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